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Immunogen
Synthesized peptide derived from human MDM2.
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Antibody s target
Human MDM2
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Antibody s full description
Rabbit Anti-Human MDM2 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ACTFS antibody, Double minute 2 protein antibody, E3 ubiquitin-protein ligase Mdm2 antibody, Hdm 2 antibody, Hdm2 antibody, HDMX antibody, MDM 2 antibody, MDM2 antibody, MDM2 oncogene E3 ubiquitin protein ligase antibody, Mdm2 p53 E3 ubiquitin protein ligase homolog antibody, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody, MDM2_HUMAN antibody, MDM2BP antibody, Mouse Double Minute 2 antibody, MTBP antibody, Murine Double Minute Chromosome 2 antibody, Oncoprotein Mdm2 antibody, p53 Binding Protein Mdm2 antibody, p53-binding protein Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody
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Verified applications
WB, IHC, ICC/IF, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total MDM2 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1329 was sufficient for detection of MDM2 in 10 µg of SKOV3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus, Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene. MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
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Bibliography
1. Uhrinova S et al. (2005) J. Mol. Biol. 350 (3): 587–98. 2. Oliner JD et al. (1992) Nature 358 (6381): 80–3. 3. Wade M. et al. (2006) J. Biol. Chem. 281 (44): 33036–44. 4. Ebrahim, M. et al. (2015) Histology and histopathology, 30(11), 1271-1282. 30(11):1271-1282.
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Released date
6/Sep/2016
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NCBI number
NP_002383.2
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Gene number
4193
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Protein number
Q00987
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Tissue: A549. Species: Human. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human SKOV3 cell lysates showing detection of ~90kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Lane 1: Human SKOV3 cells. Lane 2: Human SKOV3 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:1000. Predicted/Observed Size: ~90kDa. Rabbit Anti-MDM2 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1329) | Rabbit Anti-MDM2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on A549 (SPC-1329) | Rabbit Anti-MDM2 Antibody used in Western blot (WB) on SKOV3 cell lysates (SPC-1329)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
MDM2, APC
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Short name
Rabbit Anti- MDM2 Polyclonal: APC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
APC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman MDM2 polyclonal: adenomatous polyposis coli
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
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