RPMI 1640 w/ stable Glutamine w/ 25 mM Hepes - 500ml

• Catalog number
  LM-R1638/500
• Price
  Please ask
• Size
  40X500ml

• Description
  RPMI 1640 w/ stable Glutamine w/ 25 mM Hepes - 500ml is used in cultures in vitro, suspension and monolayer culture of human leukemic cells.
• Similar product
• Shipping conditions
  -20°C or dry ice
• Storage
  + 2 / + 8 ° C 
• Shelflife
  24 months
• State
  Liquid
• Chemical description
  Water-based mixture of inorganic and organic compounds
• Appearance
  Physical state at 20 ºC: Liquid ; Appearance: Translucent ; Colour: Red
• Density at 20 ºC
  1039 kg/m³
• Relative density at 20 ºC
  1.039
• PH
  7.3 ± 0.3
• Osmolality
  294 mOsm/kg ± 10 %
• Cell growth test
  Medium tested for the ability to support SP2/0-Ag14 cell growth.
• Composition
  NA
• Sterility
  Sterilizing filtration 
• Personal precautions
  Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
• Uses
  Usage biotechnologique. Uniquement pour usage professionnel.
• Technical data
• Data sheet
• Ordering
  To order RPMI 1640 w/ stable Glutamine w/ 25 mM Hepes - 500ml, please use the Cat. Nr.LM-R1638/500 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for RPMI 1640 w/ stable Glutamine w/ 25 mM Hepes - 500ml
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Gene target
  RPMI   1640   stable   Glutamine   500ml  
• Short name
  RPMI 1640 w/ stable Glutamine w/ 25 mM - 500ml
• Alternative name
  RPMI 1640 w/ stable Glutamine w/ 25 millimeter Hepes - 500ml

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