Mouse anti MUC-1 (CD227)

• Catalog number
  MUB2006P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Mucin 1, cell surface associated (MUC-1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in Humans. MUC-1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with colon, breast, ovarian, lung and pancreatic cancers.
• Antibody come from
  214D4 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a fusion protein, Human b-gal-episialin/MUC1 repeat domain.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  214D4
• Antigen antibody binding interaction
  Mouse anti MUC-1 (CD227) Antibody
• Antibody is raised in
  Mouse (Balb/c)
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  214D4 is directed anti the repeat domain of episialin.
• Research interest
  CD markers,Cell adhesion, Cancer
• Application
  Flow Cytometry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin)
• Antibody s suited for
  214D4 is useful for flow cytometry and immunohistochemistry on frozen and paraffin embedded tissue. Optimal antibody dilution should be determined by titration.
• Storage
  Store at 4°C, or in small aliquots at –20°C.
• Relevant references
  Hilkens, J., et al., Tumor Biol. 19 (suppl. 1): 67-70, 1998. Wesseling, J., et al., Mol. Biol. Cell 7: 565-577, 1996.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  MUC-1   CD227  
• Gene symbol
  MUC1, CD164, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
• Short name
  Mouse anti MUC-1 (CD227)
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to MUC-1 (CD227)
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:7508
• Gene
  MUC1
• Long gene name
  mucin 1, cell surface associated
• Synonyms gene
  • PUM
  • MCKD1
• Synonyms gene name
  • mucin 1, transmembrane
  • medullary cystic kidney disease 1 (autosomal dominant)
• Synonyms
  • CD227
  • PEM
  • ADMCKD
  • ADMCKD1
  • MCKD
  • MCD
  • KL-6
  • EMA
  • Ca15-3
• Synonyms name
  • epithelial membrane antigen
• GenBank acession
  • J05581
• Locus
  1q22
• Discovery year
  1986-01-01
• Entrez gene record
  4582
• Pubmed identfication
  • 1697589
  • 23396133
• RefSeq identity
  • NM_002456
• Classification
  • CD molecules
  • Mucins
• VEGA ID
  OTTHUMG00000035681

Gene info

• Identity
  HGNC:1632
• Gene
  CD164
• Long gene name
  CD164 molecule
• Synonyms gene name
  • CD164 antigen, sialomucin
  • CD164 molecule, sialomucin
• Synonyms
  • MUC-24
  • MGC-24
  • DFNA66
• Synonyms name
  • deafness, autosomal dominant 66
• GenBank acession
  • AF106518
• Locus
  6q21
• Discovery year
  2000-06-08
• Entrez gene record
  8763
• Pubmed identfication
  • 9680353
  • 9763543
  • 26197441
• RefSeq identity
  • NM_006016
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000015339
• Locus Specific Databases
  • LRG_1419

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

Gene info

• Identity
  HGNC:31545
• Gene
  MIR16-1
• Long gene name
  microRNA 16-1
• Synonyms gene
  • MIRN16-1
• Synonyms
  • hsa-mir-16-1
• Locus
  13q14.2
• Discovery year
  2004-04-23
• Entrez gene record
  406950
• RefSeq identity
  • NR_029486
• Classification
  • MicroRNA MIR15/16 family

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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