ULBP2, human recombinant

• Catalog number
  7334-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  NKG2D ligand 2, N2DL2, RAET1H
• Alternative_names
  NKG2D ligand 2, N2DL2, RAET1H
• Description
  Activates multiple signaling pathways in primary NK cells
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥85%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 microgram of protein
• Molecular Weight
  24.3 kDa (216 aa, 26-216 aa + His Tag), confirmed by MALDI-TOF.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 30% glycerol, 2 M Urea and 2 mM DTT.
• Background Information
  ULBP2, also known as NKG2D ligand 2, belongs to the MHC class I family. This protein is ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. Recombinant human ULBP2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSHMGRADP HSLCYDITVI PKFRPGPRWC AVQGQVDEKT FLHYDCGNKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI LTEQLRDIQL ENYTPKEPLT LQARMSCEQK AEGHSSGSWQ FSFDGQIFLL FDSEKRMWTT VHPGARKMKE KWENDKVVAM SFHYFSMGDC IGWLEDFLMG MDSTLEPSAG APLAMS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ULBP2  
• Gene symbol
  ULBP2
• Short name
  ULBP2, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  UL16 binding protein 2, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  UL16 binding protein 2, ULBP2 and IDBG-97842 and ENSG00000131015 and 80328, natural killer cell lectin-like receptor binding, Extracellular

Gene info

• Identity
  HGNC:14894
• Gene
  ULBP2
• Long gene name
  UL16 binding protein 2
• Synonyms
  • RAET1H
• GenBank acession
  • AF304378
• Locus
  6q25.1
• Discovery year
  2001-04-27
• Entrez gene record
  80328
• Pubmed identfication
  • 11239445
• VEGA ID
  OTTHUMG00000015803

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products