Rabbit Monoclonal IgG Anti-p63 Protein (Ab2) [EPR5701]

  • Catalog number
    PP063-G
  • Price
    Please ask
  • Size
    5 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Recombinant human p63 protein aa 1-203
  • Specificity
    p63 protein (nuclear)
  • Species reactivity
    Human, mouse, rat
  • Product presentation
    Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
  • Product category
    Primary Antibody
  • Antibody s raised in
    Rabbit
  • Antibody s isotype
    IgG
  • Antibody s clone
    EPR5701
  • Product is supplied as
    ready to use
  • Recommended concentration for use
    neat
  • Protein concentration
    N/A
  • Positive control
    Normal prostate tissue or skin, basal cell carcinoma
  • Pretreatment
    Pre-treatment of formaldehyde-fixed tissue with Tris-buffered EDTA solution pH 9 (Unmasking Fluid E, Art.-No. DE006) or TRIS/EDTA/Citrate-buffer pH 8 (Unmasking Fluid T (Art.-No. DE005) 40 min at 100°C
  • Tested applications
    IHC(P)
  • Recommended secondary reagent
    We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005). Alternatively VECTASTAIN Elite ABC (Art. No. PK-6101) or VECTOR Polymer ImmPress System (Art. No. MP-7401) will serve well.
  • Storage method
    Store at 2-8°C
  • UniProt number
    Go to UniProt/SwissProt
  • Litterature
    1. Yang A., Kaghard M., Wang Y., Gillett E., Fleming M.D., Dötsch V., et al. (1998) p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities. Mol. Cell. 2; 305-316. 2. Celli J., Duijf P., Hamel B.C.J., Bamshad M., Kramer B., Smith A.P.T., et al. (1999) Heterozygous germline mutations in p53 homolog p63 are the cause of EEC syndrome. Cell 99; 143-153. 3. Signoretti S., Waltregny D., Dilks J., Isaak B., Lin D., Garraway L., et al. (2000) p63 is a prostate basal cell marker and is required for prostate development. Am J. Pathol. 157; 1769-1775. 4. Pelosi G., Pasini F., Stenholm C.O., Pastorino U., Maisonneuve P., Sonzogni A., et al. (2002) p63 immunoreactivity in lung cancer: yet another player in the development of squamous cell carcinomas? J. Pathol. 198; 100-109. 5. Weinstein M.H., Signoretti S., and Loda M. (2002) Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod. Pathol. 15(12); 1302-1308.
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Short name
    Bioprime
  • Technique
    Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Monoclonals or monoclonal antibodies
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG, IgG
  • Alternative name
    p63 Protein (Ab2)
  • Alternative technique
    rabbit-anti, antibodies, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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