D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
D-(+)-Biotin is a coenzyme and a B vitamin. May be used to elute proteins from avidin/streptavidin resins.
Is it cell-permeable?
Is this a salt?
Protect from air and light
Maximum time for storage
White to off-white solid
DMSO (>40 mg/ml)
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
For cells, cell lines and tissues in culture till half confluency.