Mouse anti Cytokeratin 18 / Keratin K18

• Catalog number
  MUB0327P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Antibody come from
  RCK106 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with Cytokeratins from the Human bladder carcinoma cell line T24.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  RCK106
• Antigen antibody binding interaction
  Mouse anti Cytokeratin 18 / Keratin K18 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  RCK106 reacts exclusively with Cytokeratin 18 in glandular epithelial cells of the digestive, respiRatory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
• Research interest
  Cancer,Cytoskeleton
• Application
  Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
• Antibody s suited for
  RCK106 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of Keratin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of Keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to Keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Lane, E. B., Leigh, I. M., Robben, H., Huijsmans, A., Ooms, E. C., and Ruiter, D. J. (1990). Distribution of Cytokeratin polypeptides in Human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression, Am J Pathol 136, 329-43. Ivanyi, D., Groeneveld, E., Van Doornewaard, G., Mooi, W. J., and Hageman, P. C. (1990). Keratin subtypes in carcinomas of the uterine cervix: impliCations for histogenesis and differential diagnosis, Cancer Res 50, 5143-52. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511. Bauwens, L. J., De Groot, J. C., Ramaekers, F. C., Veldman, J. E., and Huizing, E. H. (1992). Expression of intermediate filament proteins in the adult Human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86. Bonfrer, J. M., Groeneveld, E. M., Korse, C. M., van Dalen, A., Oomen, L. C., and Ivanyi, D. (1994). Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantifiCation of tissue polypeptide antigen recognizes Keratin 18, Tumour Biol 15, 210-22.
• Protein number
  P05783
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Cytokeratin   Keratin   K18  
• Gene symbol
  ERVK-18
• Short name
  Mouse anti Cytokeratin 18 / Keratin K18
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Cytokeratin 18 / Keratin K18
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:39025
• Gene
  ERVK-18
• Long gene name
  endogenous retrovirus group K member 18
• Synonyms gene name
  • endogenous retrovirus group K, member 18
• Synonyms
  • c1_C
  • HERV-K18
• Synonyms name
  • HERV-K_1q23.3 provirus ancestral Env polyprotein
  • endogenous retrovirus K-18
  • IDDMK1,2-22
  • HERV-K_1q23.3 provirus ancestral Pol protein
  • HERV-K_1q23.3 provirus ancestral Pro protein
• GenBank acession
  • FN806828
  • AF012336
• Locus
  1q23.3
• Discovery year
  2011-05-05
• Pubmed identfication
  • 18664271
  • 10516026
  • 21542922

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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