CD3, epsilon (Early T Cell Marker) (FITC) Antibody
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Catalog number
GEN632607
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Price
Please ask
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Size
500ug
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Also known as
CD3, epsilon
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Other names
CD3 epsilon; N/A; CD3 epsilon; N/A; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
cd3e; N/A; N/A; N/A
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Gene name synonims
cd3e; N/A; N/A; N/A
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Other gene names
cd3e; N/A; N/A; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2b,k
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Clone
5K105
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Host organism
Mouse (Mus musculus)
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Source organism
Porcine
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Species reactivity
Porcine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes porcine CD3e, Mr 20kD.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified
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Form Appearance
Supplied as a liquid in PBS, sodium azide. Conjugated to FITC.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Description
For cells, cell lines and tissues in culture till half confluency.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
CD3, epsilon (Early T ) (FITC) Antibody
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA.
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Label
FITC
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Alternative name
Anti-CD3, epsilon (Early T Cell Marker) (FITC)
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Alternative technique
antibodies, fluorescine
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Tissue
cell
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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