pCMV-Flag-EPHA4 Plasmid

• Catalog number
  PVTB00458-2a
• Price
  380.17 USD
• Size
  2 ug

• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• Kit
  Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

• Gene target
  pCMV   EPHA4  
• Gene symbol
  EPHA4
• Short name
  pCMV- -EPHA4 Plasmid
• Technique
  plasmid, plasmids in 1
• Label
  Flag
• Alternative name
  pCMV-Flag-EPHA4 Plasmid
• Alternative technique
  plasmids

Gene info

• Identity
  HGNC:3388
• Gene
  EPHA4
• Long gene name
  EPH receptor A4
• Synonyms gene
  • TYRO1
• Synonyms gene name
  • EphA4
• Synonyms
  • Hek8
• GenBank acession
  • L36645
• Locus
  2q36.1
• Discovery year
  1992-10-02
• Entrez gene record
  2043
• Pubmed identfication
  • 9267020
• Classification
  • Sterile alpha motif domain containing
  • EPH receptors
• VEGA ID
  OTTHUMG00000133142

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