Nickel Max Flow Metal Affinity Resin, Highly Cross-linked Beads, 6%

Test

A high affinity purification column was use to purify Nickel Max Metal Resin, Highly Cross-linked , 6% by Gentaur Genprice by chromatographic size exclusion. Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

Gene target

Nickel Max Metal Resin Highly Cross-linked
Short name

Nickel Max Metal Resin, Highly Cross-linked , 6%
Technique

affinity, resin, Flow, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet.
Label

beads
Alternative name

Nickel v-myc myelocytomatosis viral oncogene homolog (avian) associated factor X Flow Metal protein Resin, very Cross-linked particles, 6%
Alternative to gene target

MYC associated factor X, bHLHd4, MAX and IDBG-9524 and ENSG00000125952 and 4149, protein dimerization activity, nuclei, Max and IDBG-151490 and ENSMUSG00000059436 and 17187, MAX and IDBG-646780 and ENSBTAG00000017994 and 540616

Name

Flow Cytometry
Concept

Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data