Biotin Conjugated Mouse Anti-rabbit IgG antibody
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Catalog number
BM2004-1
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Price
Please ask
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Size
1000 µl
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Type of the antibody protein
IgG secondary antibody
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Raised in synthesized
Mouse
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Conjugation
Biotin
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Product configuration
Solution with concentration 1mg/ml
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Immunogen
Rabbit IgG (whole molecule)
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Clonality
Polyclonal antibodies
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Analyses
IHC-P,IHC-F,ICC,WB,ELISA
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Storage conditions
Keep the Biotin Conjugated Mouse Anti-rabbit IgG antibody at plus four degrees Celsius for 12 months.
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Product details
This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all mouse serum proteins, except the specific antibody for rabbit IgG.
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Specificity
This biotin conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with human/goat/bovine IgG.
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Tips
The Biotin Conjugated Mouse Anti-rabbit IgG antibody is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by boster. Rabbit antibodies are very stable and can be stored for several days at room temperature. boster adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Conjugated Mouse Anti-rabbit IgG antibody
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Technique
Antibody, Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, IgGs, mouses
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Host
mouse, Rabbits
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Isotype
IgG, IgG
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Label
Biotin
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Species
Mouse, Mouses
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Alternative name
biotinilated coupled Mouse Antibody tohost: rabbit Immunoglobulin G (antibody to-)
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Alternative technique
antibodies, rabbit-anti, murine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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