Malate Dehydrogenase (MDH) (EC 188.8.131.52) reversibly converts L-Malate into oxaloacetate in the presence of NAD+. In eukaryotic cells, malate dehydrogenase has 2 isoforms, MDH1 and MDH2. MDH1 is cytosolic and participates in the malate-aspartate shuttle, which transports malate into the mitochondria. Malate will then be utilized for the generation of ATP. MDH2, on the other hand, is a mitochondrial enzyme that participates in the citric acid cycle. MDH2 activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). Biovision’s PicoProbeTM Malate Dehydrogenase Activity Assay Kit (Fluorometric) provides a quick and easy method for monitoring MDH activities in a wide variety of samples. In this assay, MDH converts malate into intermediate products, which will further react with PicoProbeTM forming a fluorophore (Ex/Em=535/587nm). The kit is simple, sensitive and high-throughput adaptable and can detect as low as 40 µU of MDH activity.
Simple, rapid & convenient. The assay can detect Malate Dehydrogenase (MDH) Activity less than 100 µU in various sample types.
Kit is besf for
• Measurement of malate dehydrogenase activity in various tissues/cells • Analysis of citric acid cycle and malate-asparate shuttle
Malate Dehydrogenase Activity Kit
Fluorescence (535/587 nm)
The Fluorometric PicoProbe Malate Dehydrogenase Activity Assay Kit contains MDH Assay Buffer, MDH Substrate, PicoProbeTM, MDH Developer, NADH Standard and MDH Positive Control
Animal tissues such as liver, heart, muscle, etc. Cell culture: Adherent or suspension cells Mitochondria
Store this kit at -20°C for 12 months
This product is shipped with gel pack
Please contact us to inquire the datasheet and an example certificate of analysis
This assay kit is on the market since May 2017
Biovision supplies other types of Assays as 1.
Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit
Fluorometric Malate Dehydrogenase Activity Assay Kit
fluorescence PicoProbe Malate Dehydrogenase Activity test reagent
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832