PicoProbe™ Malate Dehydrogenase Activity Assay Kit (Fluorometric)

• Catalog number
  K538-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Fluorescence(535/587 nm) • Applications- - Measurement of malate dehydrogenase activity in various tissues/cells - Analysis of citric acid cycle and malate-asparate shuttle
• Detection Method
  Fluorescence (535/587 nm)
• Species Reactivity
  Mammalian
• Applications
  • Measurement of malate dehydrogenase activity in various tissues/cells • Analysis of citric acid cycle and malate-asparate shuttle
• Sample Type
  • Animal tissues such as liver, heart, muscle, etc. • Cell culture: Adherent or suspension cells • Mitochondria
• Features Benefits
  • Simple, rapid & convenient • The assay can detect Malate Dehydrogenase (MDH) Activity less than 100 µU in various sample types.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Malate Dehydrogenase (MDH) (EC 1.1.1.37) reversibly converts L-Malate into oxaloacetate in the presence of NAD+. In eukaryotic cells, malate dehydrogenase has 2 isoforms, MDH1 and MDH2. MDH1 is cytosolic and participates in the malate-aspartate shuttle, which transports malate into the mitochondria. Malate will then be utilized for the generation of ATP. MDH2, on the other hand, is a mitochondrial enzyme that participates in the citric acid cycle. MDH2 activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). Biovision’s PicoProbeTM Malate Dehydrogenase Activity Assay Kit (Fluorometric) provides a quick and easy method for monitoring MDH activities in a wide variety of samples. In this assay, MDH converts malate into intermediate products, which will further react with PicoProbeTM forming a fluorophore (Ex/Em=535/587nm). The kit is simple, sensitive and high-throughput adaptable and can detect as low as 40 µU of MDH activity.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Conjugate
  Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit

• Gene target
  Malate   Dehydrogenase   Activity   Kit   Fluorometric  
• Short name
  ™ Malate Dehydrogenase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  PicoProbe™ Malate Dehydrogenase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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