Rabbit anti Human fibrinogen

  • Catalog number
    RAHu/Fbg
  • Price
    Please ask
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial mmunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of fibrinogen in human plasma or other body fluids or to determine its concentration. The normal concentration of fibrinogen in the blood is 2.5 to 3.5 ml /ml, but lower levels are usually adequate for haemostasis. In newborn infants the value is 1.2 to 2.4 mg/ml. Synthesis of foetal fibrinogen may persists for up to 8 weeks, when adult levels are reached. Adult level increase with age and are a risk for heart disease, myocardial infarction and stroke. Fibrinogen is an acute Phase protein and increased levels are found in loosing enteropathies, in severe malnutrition, in tissue necrosis and in malignancy. Extremely high levels are seen in acute pancreatitis and, to a lower extent, in nephritic syndrome. A moderate raise may also been seen during pregnancy and the use of oral contraceptives. Fibrinogen deficiency may be congenital or acquired. If sufficiently severe, it may result in a bleeding disorder. The congenital form is very rare. Acquired hypofibrinogaemia is relatively common, probably indicating increased consumption during intravascular clotting.
  • Antibody come from
    Fibrinogen (clotting factor I) is a heat labile beta glycoprotein (molecular weight 340,000) and consists of three pairs of chains bound by disulphide bonds. It is synthesized in hepatocytes under genetic control. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis, releasing the fibrinopeptides A and B (molecular weight 50,000-65,000) and forming fibrin monomers. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking under the influence of factor XIII. The predominant gamma chain of normal fibrinogen (MW 50,000, with higher variants) has a low affinity for platelet binding. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.
  • Clone
    Polyclonal
  • Antigen antibody binding interaction
    Rabbit anti Human fibrinogen Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    The antiSerum does not cross react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
  • Antibody s specificity
    Precipitating polyclonal Rabbit antiSerum to Human fibrinogen.
  • Research interest
    Immunology
  • Application
    Immunoprecipitation
  • Antibody s suited for
    Immunoprecipitation. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Precipitin titre 1:16 when tested against pooled normal human plasma in agar-block immunodiffusion titration.
  • Storage
    Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
  • Relevant references
    no information yet
  • Protein number
    P02671
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Gene symbol
    FGG, FGB, FGA
  • Short name
    Rabbit anti fibrinogen
  • Technique
    anti human, Rabbit, anti, antibody to, anti human antibodies
  • Host
    Rabbit, Rabbits
  • Isotype
    not specified
  • Label
    unlabeled
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit antibody to H. sapiens fibrinogen
  • Alternative technique
    rabbit-anti, antibodies
Gene info
Gene info
  • Identity
  • Gene
    FGB
  • Long gene name
    fibrinogen beta chain
  • Synonyms gene name
    • fibrinogen, B beta polypeptide
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Fibrinogen C domain containing
    • Receptor ligands
  • VEGA ID
  • Locus Specific Databases
Gene info
  • Identity
  • Gene
    FGA
  • Long gene name
    fibrinogen alpha chain
  • Synonyms gene name
    • fibrinogen, A alpha polypeptide
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Fibrinogen C domain containing
    • Receptor ligands
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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