Rabbit Anti-Rat UT-A1 Polyclonal: FITC

• Catalog number
  SPC-406D-FITC
• Price
  Please ask
• Size
  100 µg

• Immunogen
  Produced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)
• Antibody s target
  Rat UT-A1
• Antibody s full description
  Rabbit Anti-Rat UT-A1 Polyclonal
• Primary research fields
  Neuroscience, Pumps/Transporters, Urea Transporters
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  SLC14A2 Antibody, FLJ16167 Antibody, hUT-A6 Antibody, HUT2 Antibody, kidney Antibody, MGC119566 Antibody, MGC119567 Antibody, Slc14a2 Antibody, Solute carrier family 14 (urea transporter) Antibody, member 2 Antibody, Solute carrier family 14 member 2 Antibody, Urea transporter 2 Antibody, Urea transporter Antibody, Urea transporter kidney Antibody, UT-A2 Antibody, UT2 Antibody, UT2_HUMAN Antibody, UTA Antibody, UTR Antibody, UT1 Antibody, UTA1 Antibody
• Verified applications
  WB, ICC/IF
• Raised in
  Rabbit
• Antibody s reactivity
  Rat, Mouse
• Antibody s recommended dilutions for use
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Protein A Purified
• Recommended buffer for storage
  PBS, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~97 and 127kDa.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  1 µg/ml of SPC-406 was sufficient for detection of UT-A1 in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane, Apical Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service.
• Scientific context
  UT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates trans-epithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promoter and expressed in the thin descending limb of Henle, viz. UT-A2) (1, 2).
• Bibliography
  1. Nielsen S., et al. (1996) Proc Natl Acad Sci U S A. 93(11): 5495-500. 2. Smith C.P. (2009) Exp Physiol. 94(2): 180-185.
• Released date
  11/Jun/2010
• NCBI number
  NP_062220
• Gene number
  54302
• Protein number
  Q62668
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
• Representative figure legend
  Western blot analysis of Rat Inner medulla showing detection of UT-A1 protein using Rabbit Anti-UT-A1 Polyclonal Antibody (SPC-406). Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody (SPC-406) at 1:1000. Rabbit Anti-UT-A1 Antibody used in Western blot (WB) on Rat Inner medulla (SPC-406)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Oryctolagus cuniculus, Rattus norvegicus

• Gene target
  UT-A1  
• Gene symbol
  RFC4, RFC2, SNAR-A1, PCDHGA1, ZNF2, SCAF1, HNRNPA1, FALEC, RFC1, EDA
• Short name
  Rabbit Anti- UT-A1 Polyclonal: FITC
• Technique
  Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  production species: rabbit Antibody toRat UT-A1 polyclonal: fluorecein
• Alternative technique
  polyclonals, rabbit-anti, antibodies, fluorescine

Gene info

• Identity
  HGNC:9972
• Gene
  RFC4
• Long gene name
  replication factor C subunit 4
• Synonyms gene name
  • replication factor C (activator 1) 4 (37kD)
  • replication factor C (activator 1) 4, 37kDa
• Synonyms
  • A1
  • RFC37
• Synonyms name
  • A1 37 kDa subunit
  • activator 1 37 kDa subunit
  • RFC 37 kDa subunit
• Locus
  3q27.3
• Discovery year
  1994-10-14
• Entrez gene record
  5984
• Pubmed identfication
  • 7774928
• RefSeq identity
  • NM_002916
• Classification
  • AAA ATPases
• VEGA ID
  OTTHUMG00000156515

Gene info

• Identity
  HGNC:9970
• Gene
  RFC2
• Long gene name
  replication factor C subunit 2
• Synonyms gene name
  • replication factor C (activator 1) 2 (40kD)
  • replication factor C (activator 1) 2, 40kDa
• Synonyms
  • A1
  • RFC40
• Synonyms name
  • activator 1
• Locus
  7q11.23
• Discovery year
  1994-10-14
• Entrez gene record
  5982
• Pubmed identfication
  • 1313560
  • 7774928
• RefSeq identity
  • NM_181471
• Classification
  • AAA ATPases
• VEGA ID
  OTTHUMG00000023239

Gene info

• Identity
  HGNC:34304
• Gene
  SNAR-A1
• Long gene name
  small NF90 (ILF3) associated RNA A1
• Synonyms gene name
  • small nuclear ILF3/NF90-associated RNA A1
  • small ILF3/NF90-associated RNA A1
• Synonyms
  • SNAR-A53113498
• GenBank acession
  • EU071051
• Locus
  19q13.33
• Discovery year
  2008-06-20
• Entrez gene record
  100126798
• Pubmed identfication
  • 17855395
• RefSeq identity
  • NR_004435
• Classification
  • Small NF90 (ILF3) associated RNAs

Gene info

• Identity
  HGNC:8696
• Gene
  PCDHGA1
• Long gene name
  protocadherin gamma subfamily A, 1
• Synonyms
  • PCDH-GAMMA-A1
• GenBank acession
  • AF152318
• Locus
  5q31
• Discovery year
  2000-06-28
• Entrez gene record
  56114
• Pubmed identfication
  • 10380929
• RefSeq identity
  • NM_018912
• Classification
  • Clustered protocadherins
• VEGA ID
  OTTHUMG00000163678

Gene info

• Identity
  HGNC:12991
• Gene
  ZNF2
• Long gene name
  zinc finger protein 2
• Synonyms gene name
  • zinc finger protein 2 (A1-5)
• Synonyms
  • A1-5
  • ZNF661
  • Zfp661
• GenBank acession
  • X60152
• Locus
  2q11.1
• Discovery year
  1989-05-31
• Entrez gene record
  7549
• Pubmed identfication
  • 8183940
  • 1945843
• RefSeq identity
  • NM_021088
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000155150

Gene info

• Identity
  HGNC:30403
• Gene
  SCAF1
• Long gene name
  SR-related CTD associated factor 1
• Synonyms
  • SR-A1
  • FLJ00034
• GenBank acession
  • AK024444
• Locus
  19q13.3-q13.4
• Discovery year
  2007-08-09
• Entrez gene record
  58506
• Pubmed identfication
  • 11461075
• RefSeq identity
  • NM_021228
• VEGA ID
  OTTHUMG00000183238

Gene info

• Identity
  HGNC:5031
• Gene
  HNRNPA1
• Long gene name
  heterogeneous nuclear ribonucleoprotein A1
• Synonyms gene
  • HNRPA1
• Synonyms
  • hnRNP-A1
  • ALS20
• GenBank acession
  • BC009600
• Locus
  12q13.13
• Discovery year
  1991-05-21
• Entrez gene record
  3178
• Pubmed identfication
  • 1733858
  • 23455423
• RefSeq identity
  • NM_031157
• Classification
  • RNA binding motif containing
  • Spliceosomal A complex
  • Heterogeneous nuclear ribonucleoproteins
• VEGA ID
  OTTHUMG00000169702

Gene info

• Identity
  HGNC:43713
• Gene
  FALEC
• Long gene name
  focally amplified long non-coding RNA in epithelial cancer
• Synonyms gene
  • LINC00568
• Synonyms gene name
  • long intergenic non-protein coding RNA 568
• Synonyms
  • ncRNA-a1
  • FAL1
• Synonyms name
  • focally amplified lncRNA on chromosome 1
• GenBank acession
  • AL713297
• Locus
  1q21.2
• Discovery year
  2012-02-02
• Entrez gene record
  100874054
• Pubmed identfication
  • 20887892
  • 25203321
  • 29196104
• RefSeq identity
  • NR_051960
• Classification
  • Long non-coding RNAs with non-systematic symbols
• VEGA ID
  OTTHUMG00000035849

Gene info

• Identity
  HGNC:9969
• Gene
  RFC1
• Long gene name
  replication factor C subunit 1
• Synonyms gene name
  • replication factor C (activator 1) 1 (145kD)
  • replication factor C (activator 1) 1, 145kDa
• Synonyms
  • A1
  • PO-GA
  • RFC140
  • MHCBFB
• GenBank acession
  • L23320
• Locus
  4p14
• Discovery year
  1994-10-14
• Entrez gene record
  5981
• Pubmed identfication
  • 8114700
• RefSeq identity
  • NM_002913
• Classification
  • AAA ATPases
• VEGA ID
  OTTHUMG00000099363

Gene info

• Identity
  HGNC:3157
• Gene
  EDA
• Long gene name
  ectodysplasin A
• Synonyms gene
  • ED1
  • EDA2
  • ODT1
• Synonyms gene name
  • ectodermal dysplasia 1, anhidrotic
  • oligodontia 1
• Synonyms
  • EDA1
  • XLHED
  • HED
  • XHED
  • ED1-A1
  • ED1-A2
  • EDA-A1
  • EDA-A2
• GenBank acession
  • U59227
• Locus
  Xq13.1
• Discovery year
  2001-06-22
• Entrez gene record
  1896
• Pubmed identfication
  • 8696334
  • 18657636
  • 16583127
• RefSeq identity
  • NM_001399
• Classification
  • MicroRNA protein coding host genes
  • Tumor necrosis factor superfamily
• VEGA ID
  OTTHUMG00000021764
• Locus Specific Databases
  • Global Variome shared LOVD

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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