BD-1, human recombinant
-
Catalog number4506-1000
-
PricePlease ask
-
Size1 mg
-
-
Synonymsβ-defensin 1, BD-1, Defensin β 1, hBD-1, HBD1, HBP1, DEFB1, HBD-1, HBP-1, DEFB101, DEFB-1, MGC51822
-
Alternative_namesβ-defensin 1, BD-1, Defensin β 1, hBD-1, HBD1, HBP1, DEFB1, HBD-1, HBP-1, DEFB101, DEFB-1, MGC51822
-
DescriptionAn antimicrobial peptide
-
RecombinantYes
-
SourceE. coli
-
Purity by SDS PAGE≥98%
-
AssaySDS-PAGE
-
Biological activityDetermined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1 µg/ml.
-
Results0.1-1 µg/ml
-
Molecular Weight5.0 kDa
-
Storage Temp-20°C
-
ShippingGel pack
-
Shelf Life12 months
-
AppearanceLyophilized protein
-
Physical form descriptionLyophilized from a concentrated (1 mg/ml) solution containing 20 mM PBS pH-7.4 and 130 mM sodium chloride
-
Reconstitution InstructionsReconstitute in dH₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
-
Background InformationThe Defensin family are highly similar in their protein sequence and are microbicidal & cytotoxic peptides made by neutrophils. β Defensin-1 (BD-1) is an antimicrobial peptide having the resistance of epithelial surfaces to microbial colonization. BD-1 has close proximity to Defensin α-1 and has been implicated in the pathogenesis of cystic fibrosis. Skin of patients having atopic dermatitis patients and mycosis fungoides (non-lesional and lesional) show lower human BD-1 mRNA expression and higher human BD-2 and human BD-3 mRNA expression. β Defensin is highly expressed by epithelial cells. BD-1 may play a role in the pathogenesis of severe sepsis. Variation in human BD-1 contributes to asthma diagnosis, with apparent gender-specific effects. Human BD-3 is a dimer, while Human BD-1 and Human BD-2 are monomeric. Recombinant human β Defensin-1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of 5 kDa.
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional sourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolDEFB1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
-
Short nameBD-1, recombinant
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
SpeciesHuman, Humans
-
Alternative nameBD-1, H. sapiens Rec.
-
Alternative techniquerec
-
Gene info
-
Identity
-
Gene
-
Long gene namedefensin beta 1
-
Synonyms gene name
- defensin, beta 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1997-05-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Defensins, beta
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1/206 family
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 8-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 7-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
-
Synonyms gene
-
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Immunoglobulin kappa (IGK) orphons
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1289-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2008-11-03
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1289 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 101-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR101 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 16-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR15/16 family
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
