Flotillin 2 Antibody
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Catalog numberR31143
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenFlotillin 2
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting amounts. Titration of the Flotillin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis Flotillin 2 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ14254
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PurityAntigen affinity
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DescriptionFlotillin 2, also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al.(1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the FLOT2 cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al.(1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al.(2012) found that palmitoylation and oligomerization of Flotillin2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of Flotillin2 was not changed in Dhhc5 mutant neurons.
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ImmunogenAn amino acid sequence from the N-terminus of human FLOT2 (TLTVEQIYQDRDQFA) was used as the immunogen for this Flotillin 2 antibody (100% homologous in human, mouse and rat).
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StorageAfter reconstitution, the Flotillin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolFLOT2
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Short nameAnti-Flotillin 2
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to Flotillin 2
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameflotillin 2
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-05-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Flotillins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data