FastAmp™ Plant Direct PCR Kit - 1,250 Reactions

Description

Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps. The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmpTM Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis. Highlights: Direct PCR- no need to purify DNA Specially engineered Taq DNA polymerase with highest sensitivity and specificity Extremely short PCR protocol times Master mix format with premixed gel loading dye to reduce cross-contamination and sample handling errors 5x magic enhancer for high GC containing DNA amplification Product Includes: FastAmp™ plant direct PCR master mix (2x) Dilution buffer Control primer mix (25 µM each) 5x magic enhancer Nuclease- free water
Shipping Condition

Ships with Dry Ice
Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Plants RNA, DNA , cDNA and plant tissues are used to study plant genes.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Gene target

FastAmp™ Direct Kit 250
Short name

FastAmp™ Direct PCR Kit - 1 250
Technique

PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Species

Plants
Alternative name

FastAmp™ vegetal Direct PCR test kit reagent - 1,250 Reactions
Alternative technique

kits, dna-amplification
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data