GAPDH Antibody
-
Catalog number3777R-100
-
PricePlease ask
-
Size100 µg
-
-
Antibody TargetGAPDH
-
Target Alternative NameGAPDH, G3PD, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
-
Antibody TypePolyclonal
-
Species ReactivitiesHuman, mouse, rat
-
Immunogen SequenceSynthetic peptide surrounding amino acid 317of rat GADPH
-
AccessionXP_214281.3
-
Gene Id290604
-
AppearanceColorless liquid
-
FormLiquid
-
Concentration0.5 mg/ml
-
Formulation100µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
-
PurificationAffinity purified
-
ApplicationWestern blot
-
Application And UsagesWestern blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes over 100 kDa band corresponding to NIF-1 in samples from human origin. Reactivity to other species has not been tested.
-
DescriptionGlyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the b-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins.
-
Storage Condition-20 °C
-
Shipping Conditiongel pack
-
Shelf Life12 months
-
UsageFor Research Use Only! Not to be used in humans.
-
HandlingThe antibody solution should be gently mixed before use.
-
Western Blot VerifiedVerified
-
Immunocytochemistry VerifiedNot Verified
-
Immunofluorescence VerifiedNot Verified
-
Immunoprecipitation VerifiedNot Verified
-
FACS VerifiedNot Verified
-
ELISA VerifiedNot Verified
-
ChIP VerifiedNot Verified
-
Flow Cytometry VerifiedNot Verified
-
PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolGAPDH-DT, GAPDH, GAPDHS, GAPDHP15
-
Short nameGAPDH Antibody
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative nameGAPDH (antibody to-)
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameGAPDH divergent transcript
-
Locus
-
Discovery year2021-03-12
-
Entrez gene record
-
Classification
- Divergent transcripts
Gene info
-
Identity
-
Gene
-
Long gene nameglyceraldehyde-3-phosphate dehydrogenase
-
Synonyms gene
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameglyceraldehyde-3-phosphate dehydrogenase, spermatogenic
-
Synonyms gene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2004-02-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameglyceraldehyde-3-phosphate dehydrogenase pseudogene 15
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2003-11-26
-
Entrez gene record
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data