Anti-14-3-3 gamma (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-12421R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein Peptide14-3-3 gamma
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SpecificityThis antibody reacts specifically with 14-3-3 gamma
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human 14-3-3 gamma
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Gene ID7532
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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Synonyms14 3 3 protein gamma; 14 3 3 protein gamma subtype; 14 3 3gamma; 14-3-3 protein gamma; 1433G_HUMAN; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide; KCIP 1; KCIP-1; KCIP1; N-terminally processed; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide; YWHAG.
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Also known as14-3-3 gamma Polyclonal Antibody
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Other nameAnti-14-3-3 gamma Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolYWHAQ, YWHAG, YWHAZ, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12
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Short nameAnti-14-3-3 gamma
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative name14-3-3 gamma Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
- protein, theta
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
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Synonyms gene
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 14
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 12
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data