Human CellExp™ Osteoactivin / GPNMB, human recombinant

  • Catalog number
    7513-10
  • Price
    Please ask
  • Size
    10 ug
  • Synonyms
    GPNMB, HGFIN, NMB, Osteoactivin
  • Alternative_names
    GPNMB, HGFIN, NMB, Osteoactivin
  • Description
    ≥95% pure, active, human cell expressed, human recombinant Osteoactivin / GPNMB - acts as a downstream mediator of BMP-2.
  • Recombinant
    Yes
  • Source
    HEK293 cells
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 4 - 16 µg/ml.
  • Results
    ED50 for this effect is typically 4 - 16 µg/ml
  • Molecular Weight
    This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 52.9 kDa. The predicted N-terminus is Ala 22. DTT-reduced Protein migrates as 85-100 kDa due to glycosylation.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized from 0.22 um filtered solution in PBS, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
  • Background Information
    Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain. GPNMB is a transmembrane glycoprotein that is up-regulated in various cancer cells, including in glioblastoma multiforme and is expressed in many melanoma cells, as well as in tissue macrophages. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    GPNMB
  • Short name
    CellExp™ Osteoactivin / GPNMB, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ Osteoactivin / glycoprotein (transmembrane) nmb, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    glycoprotein (transmembrane) nmb, HGFIN and NMB, GPNMB and IDBG-9627 and ENSG00000136235 and 10457, heparin binding, Plasma membranes, Gpnmb and IDBG-146080 and ENSMUSG00000029816 and 93695, BT.99652 and IDBG-630320 and ENSBTAG00000000604 and 509600
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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