15-hydroxyprostaglandin dehydrogenase (15-PGDH, EC: 22.214.171.124) catalyzes the oxidation of Prostaglandins (PG) to 15-keto metabolites which show greatly reduced biological activity when compared to Prostaglandins. 15-PGDH catalyzes the rate-limiting step of PG catabolism. More recently, the inhibition of 15-PGDH has been reported as a negative regulator of tissue repair and inhibition of this enzyme can potentiate tissue regeneration in mouse models. Thus, pharmacological inhibition of 15-PGDH could have clinical implications by accelerating potential tissue regeneration after damage. In this assay, 15-PG is oxidized by 15-PDGH generating 15-Keto metabolites and oxidizing NAD. NADH reduces a Fluorometric probe thus, generating an enhanced and stable fluorescence signal (Ex/Em=535/587 nm). In the presence of an inhibitor, the enzymatic activity is inhibited resulting No/Low fluorescence. BioVision’s 15-PGDH Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of 15-PGDH inhibitors.
• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Applications: Screening/studying/characterizing 15:PGDH inhibitors.
• Simple method to screen 15-PGDH inhibitors • High-throughput Compatible • Includes 15-PGDH Inhibitor Control,
Signal Transduction, 15-PGDH
Kit's other name
Prostaglandin-Endoperoxide Synthase 2 Inhibitor Screening Kit (Fluorometric), PTGS2 Inhibitor Screening Kit (Fluorometric)
• 15-PGDH Assay Buffer • PicoProbeTM Probe (in DMSO) • 15-PGDH Developer • 15-PGDH Substrate (in DMSO) • 15-PGDH Enzyme • 15-PGDH Inhibitor Control (in DMSO)
Maximum time can be stored
Screening/studying/characterizing 15-PGDH inhibitors.
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
15-PGDH Inhibitor Screening Kit (Fluorometric)
15-PGDH suppressor quantification reagent (fluorescence)
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832