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Granzyme B human

Granzyme B human is available 2 times from Biovision labs

K4279-100 | Granzyme B (human) ELISA Kit size: 100 assays | 924.93 USD

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4728-5 | Granzyme B, human recombinant (Insect) size: 5 ug | 467.14 USD

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Summary • Detection method- Absorbance (450 nm) • Species reactivity-mouse • Application- Quantitative protein detection, establishing normal range, validation of antibody array results
Detection Method Absorbance (450 nm)
Species Reactivity human
Applications This ELISA kit is used for quantitative protein detection, establishing normal range
Sample Type • Serum & plasma (heparin, EDTA) • Cell culture supernatants
Features & Benefits • Easy, convenient and time-saving method to assay for human Granzume B. • Detection Range:15.6 pg/ml – 1000 pg/ml. Sensitivity: < 10 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.
Storage Conditions -20°C
Shipping Conditions gel pack
Shelf life 12 months
Background Granzyme B is a serine protease expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and functions in cell-mediated immunity. BioVision’s human Granzyme B ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human Granzyme B is coated on a 96-well plate. Standards and test samples are added to the wells and Granzyme B present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Granzyme B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Granzyme B captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 10 pg/ml.
Synonyms GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11
Alternative_names GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11
Recombinant Yes
Source Insect cells
Purity by SDS-PAGE ≥90%
Assay SDS-PAGE
Biological activity Activity of GZMB was tested using the synthetic peptide, IETD-AFC, as a substrate. GZMB releases AFC and then the GZMB activity can be quantified by fluorescent detection of free AFC at Ex/Em=380/500 nm.
Results ≥40 units/ug
Molecular Weight 32 kDa
Storage Temp. -70°C
Shipping gel pack
Shelf Life 12 months
Appearance Lyophilized protein
Physical form description Lyophilized powder from 0.22 um filtered solution in PBS containing 8% trehalose
Reconstitution Instructions Use 50 µl sterile D.I. water to dissolve 5 µg protein to a final conc of 0.1 µg protein/µl.
Background Information Granzyme B (GZMB) is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
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