Phospho-SMAD3 Antibody (pS213)
-
Catalog number
F48450-0.4ML
-
Price
Please ask
-
Size
0.4 ml
-
-
Category
Antibody
-
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
-
Form
Antigen affinity purified
-
Conjugation
Unconjugated
-
Clone
Polyclonal antibody
-
Recognised antigen
Phospho-SMAD3 (pS213)
-
Host animal
Rabbit (Oryctolagus cuniculus)
-
Clonality
Polyclonal (rabbit origin)
-
Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applications
IHC, WB, ELISA
-
Recommended dilutions
IHC (Paraffin): 1:50-1:100,Western blot: 1:1000
-
Notes
Titration of the phospho-SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented use
This phospho-SMAD3 antibodyis to be used only for research purposes and not for diagnostics..
-
Uniprot
P84022
-
Purity
Antigen affinity
-
Description
SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.
-
Immunogen
This phospho-SMAD3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS213 of human SMAD3.
-
Storage
Aliquot the phospho-SMAD3 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
-
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
-
Gene target
-
Gene symbol
SMAD3
-
Short name
Anti-Phospho-SMAD3 (pS213)
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Isotype
Rabbit Ig
-
Alternative name
Antibodies to Phospho-SMAD3 (pS213)
-
Alternative technique
antibodies
-
Alternative to gene target
SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125
-
Gene info
-
Identity
-
Gene
-
Long gene name
SMAD family member 3
-
Synonyms gene
-
Synonyms gene name
- MAD, mothers against decapentaplegic homolog 3 (Drosophila)
- SMAD, mothers against DPP homolog 3 (Drosophila)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1996-11-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products