NAD Kinase (catalytic domain), human recombinant
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Catalog number
7559-10
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Price
Please ask
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Size
10 ug
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Synonyms
NADK; Poly (P)/ATP NAD Kinase; EC 2.7.1.23.
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Alternative_names
NADK; Poly (P)/ATP NAD Kinase; EC 2.7.1.23.
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Description
Catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
≥ 2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1 µmol of NADP per min.
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Results
≥ 2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1 µmol of NADP per min.
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Unit Definition
One unit is defined as the amount of enzyme that synthesizes 1 µmol of NADP per min.
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Molecular Weight
~42 kDa (monomer). Human NAD kinase (aa 64-446) is fused at the N-terminus to a His-tag.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
6 months
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Concentration
1 mg/ml
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Appearance
Liquid
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Physical form description
1 mg/ml of ammonium sulphate suspension.
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Background Information
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
NAD Kinase (catalytic domain), recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Nicotinamide adenine dinucleotide phosphorylation catalyst (catalytic domain), H. sapiens Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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