NAD Kinase (catalytic domain), human recombinant

• Catalog number
  7559-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  NADK; Poly (P)/ATP NAD Kinase; EC 2.7.1.23.
• Alternative_names
  NADK; Poly (P)/ATP NAD Kinase; EC 2.7.1.23.
• Description
  Catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  ≥ 2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1 µmol of NADP per min.
• Results
  ≥ 2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1 µmol of NADP per min.
• Unit Definition
  One unit is defined as the amount of enzyme that synthesizes 1 µmol of NADP per min.
• Molecular Weight
  ~42 kDa (monomer). Human NAD kinase (aa 64-446) is fused at the N-terminus to a His-tag.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  6 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml of ammonium sulphate suspension.
• Background Information
  NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NAD   Kinase   catalytic   domain  
• Short name
  NAD Kinase (catalytic domain), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Nicotinamide adenine dinucleotide phosphorylation catalyst (catalytic domain), H. sapiens Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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