-
Alternative name1
Anti-Goat F(ab')2 Anti-Rat IgG (H+L), Mouse ads-LE/AF
-
Alternative name2
Caprine F(ab')2 Anti-Rat IgG (H+L), Murine ads-LE/AF
-
Alternative name3
Caprinae F(ab')2 Anti-Rattus norvegicus IgG (H+L), Mus musculus ads-LE/AF
-
Alternative name4
Goat F(ab')2 Anti-Rat IgG (H+L chain specific)
-
Alternative name5
Goat F(ab')2 Anti-Rat IgG(H+L), Mouse ads - Low Endotoxin/Azide-Free; Goat F(ab')2 Anti-Rat IgG (H+L chain specific) (Absorbed against mouse immunoglobulins)
-
Other names
N/A
-
Gene name
N/A
-
Gene name synonims
N/A
-
Other gene names
N/A
-
Category
Secondary Antibodies
-
Clonality
Polyclonal
-
Immunoglobulin isotype
IgG
-
Clone
Polyclonal antibody
-
Host organism
Goat
-
Species reactivity
Rat
-
Specificity and cross reactivity
Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with mouse immunoglobulins.
-
Purification method
Gel filtration of pepsin-digested antibody.
-
Form Appearance
Low Endotoxin, Azide - Free (LE/AF)
-
Concentration
N/A
-
Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) and Texas Red (TXRD) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at The items should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Texas Red (R-PE-TXRD), and R-phycoerythrin- Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at The items should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store productone at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product productone should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
-
Tested for
Direct and Indirect Immunofluorescent Staining; ELISA; FLISA; Immunoblot; Immunohistochemistry
-
Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
-
Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Group
f(ab')2s
-
Latin name
Capra aegagrus hircus, Mus musculus, Rattus norvegicus
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Gene target
-
Gene symbol
FUT3, HPS4, NPAS4
-
Short name
Goat F(ab')2 Anti- IgG (H+L), Mouse ads-LE/AF
-
Technique
F(ab')2, anti-, Mouse, anti, Goat, IgG, antibody to, antibodies, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. goats, IgGs, mouses
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Isotype
IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
-
Species
Mouse, Goats, Mouses, Rats
-
Alternative name
caprine F(Antibody')2 Antibody toRat Immunoglobulin G (heavy + light chain), Mouse ads-LE/AF
-
Alternative technique
secondairy, murine, antibodies, caprine, immunoglobulins
-
