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Category
Primary Antibodies
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Long description
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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Antibody come from
LL020 is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 murine myeloma cells with spleen cells from a Mouse immunized with a carboxyterminal peptide of Cytokeratin 6.
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Other description
Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
LL020
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Antigen antibody binding interaction
Mouse anti Cytokeratin 6 / Keratin K6 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human,Zebrafish
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
Keratin 6 is specifically found in hyperprolifeRative squamous epithelium such as psoriatic lesions and squamous cell carcinomas, e.g. of the lung. It is expressed in suprabasal Keratinocytes of wounded epidermis, a situation of high cell turnover. LL020 reacts with the basal and suprabasal layers of the entire outer root sheath in normal skin.
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Research interest
Cytoskeleton
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Application
not tested yet / to be tested
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Antibody s suited for
LL020 can be used as a prolifeRation marker in hyperprolifeRative malignant tissue, such as squamous cell carcinoma of the lung. LL020 is suitable for immunoblotting and for immunohistochemistry on frozen tissue and paraffin embedded tissue. Optimal antibody dilution should be determined by titration.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
Markey, A.C., Lane, E.B., Macdonald, D.M., Leigh, I.M. (1992). Keratin expression in basal cell carcinomas. Br. J. Dermatol. 126, 154-160. Hughes, B.R., Morris, C., Cunliffe, W.J., Leigh, I.M. (2008). Keratin expression in pilosebaceous epithelia in truncal skin of acne patients. Br. J. Dermatol. 134, 247-56. Van der Velden, L.A., Schaafsma, H.E., Ramaekers, F.C. and Kuijpers, W. (1993). Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview. Head Neck 15, 133-46. Poschmann, G., Sitek, B., Sipos, B., Ulrich, A., Wiese, S., Stephan, C., Warscheid, B., Klöppel, G., Vander Borght, A., Ramaekers, F.C.S., Meyer, E. and Stühler, K. (2009). IdentifiCation of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium. Molecular & Cellular proteomics 8, 1105-16.
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Protein number
P02538, P04259, P48668
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
