anti-Cdc25B (phospho Ser149) Antibody
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Catalog number
79-514
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Price
Please ask
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Size
0.1 mg
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Gene info
Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
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Gene names
AI604853, CDC25B
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Antibodie s label
unlabelled serum
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Purity
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
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Ab raised in
Rabbit
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Type
Polyclonal
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Spicific to
This antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.
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Antigen
Cdc25B (Phospho-Ser149) antibody was made against a peptide sequence around phosphorylation site of Serine 149 (F-R-S (p) -L-P) derived from Mouse Cdc25B.
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Immuno application
Western Blot
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Antibody advice
Western Blot: 1:500~1:1000
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Suspention pH azide
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Keep at
Store antibody at -20 grades C for up to one year.
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Works with
Mouse, Rat
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Omim nr
161621261
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Ncbi nr
NP_001104545.1
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KDa
62 kDa
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Vial with antibody
in solution
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Quantity per ml
1 mg/mL
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NCBI GENE nr
12531
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GENE symbol
Cdc25b
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Antigene name
cell division cycle 25B
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Source
Murine
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Protein nr
Q9DBN8
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
anti-Cdc25B (phospho Ser149) Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toCdc25B (phosphorilated Ser149) (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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