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Availability
Please contact us to check the availability
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Immunogen
Purified human IgG4
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Specificity
human IgG4
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Species reactivity
Human
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Product presentation
Affinity purified antibody in GBS, pH7.4, 0,099% NaN3, 0,1% EACA, 1 mM EDTA, 0,01% Benzamidine
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Product category
Primary Antibody
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Antibody s raised in
Sheep
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Antibody s isotype
IgG
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Antibody s clone
polyclonal
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:20
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Protein concentration
0,1 mg/ml
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Tested applications
IEP, WB, ELISA, Immunohistochemistry
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Storage method
Store at 2-8°C
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Litterature
1. Kleger A, Seufferlein T, Wagner M, Tannapfel A, Hoffmann TK, Mayerle J (2015) IgG4-associated autoimmune diseases—polymorphous presentation complicates diagnosis and treatment. Dtsch Arztebl Int; 112: 128–35. 2. Stone et al. (2012). Recommendations for the nomenclature of IgG4-related disease and its individual organ system manifestations. Arthritis & Rheumatism. 64 (10): 3061–3067. 3. Deshpande et al. (2012). Consensus statement on the pathology of IgG4-related disease. Modern Pathology. 25 (9): 1181–1192. 4. A. Fernandez-Flores (2012) The role of IgG4 in cutaneous pathology. Rom J Morphol Embryol, 53(2):221-231.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Group
Polyclonals and antibodies
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Gene target
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Short name
Research
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Technique
Polyclonal, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Sheep
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Isotype
IgG, IgG
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Species
Human, Humans, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
IgG4, human
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Alternative technique
polyclonals, antibodies, immunoglobulins
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