Target of this antibody:
Alternative names of antibody target:
Hydroxyacyl-coenzyme A dehydrogenase
L-3-hydroxyacyl-CoA dehydrogenase (HADH/HCDH) is a mitochondrial matrix protein expressed in pancreas, liver, heart, kidney and skeletal muscle. L-3-hydroxyacyl-CoA dehydrogenase catalyzes the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene can lead to HADH (3-α-hydroxyacyl-CoA dehydrogenase) deficiency and familial hyperinsulinemic hypoglycemia 4 (HHF4).
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 305 of rat HADH/HCDH
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-HADH/HCDH polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide (Cat.# 3264BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.