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Human Recombinant MDH2

Human Recombinant MDH2 is available 1 time from Biovision labs

6356-50 | Human Recombinant MDH2 size: 50 ug | 525.92 USD

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Synonyms Human Recombinant MDH2
Alternative_names Malate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MOR1
Description Involved in oxidation of malate to OAA
Recombinant Yes
Source E. Coli
Purity by SDS-PAGE ≥95%
Assay SDS-PAGE
Biological activity Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.
Unit Definition Specific activity is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.
Molecular Weight 35.2 kDa (335 aa, 25-338 aa + NT His Tag)
Storage Temp. -20°C
Shipping gel pack
Shelf Life 12 months
Concentration 1 mg/ml
Appearance Liquid
Physical form description 1 mg/ml solution in 20 mM Tris-HCl (pH 7.5) containing 10% glycerol.
Background Information MDH2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH2 is localized to the mitochondria and takes part in the malate-aspartate shuttle that functions in the metabolic coordination between cytosol and mitochondria. MDH2 is highly expressed in the adrenal system, small intestine, heart and pancreas.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetMDH2
Short name Recombinant MDH2
Technique Recombinant
Species Human
Alternative name H. sapiens recombinant MDH2
Alternative technique rec
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