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Antibody Target
NFkB p65
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Target Alternative Name
RELA, NFKB3, mgC131774
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Antibody Type
Polyclonal
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Species Reactivities
Human, mouse, rat
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Immunogen Sequence
Synthetic peptide surrounding amino acid 274 of mouse NFkB p65
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Accession
NP_033071
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Gene Id
19697
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
0.2 mg/ml
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Formulation
100 µg (0.2 mg/ml) affinity purified rabbit anti-NFkB polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 25% glycerol, 0.5% BSA, 0.01% thimerosal.
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Purification
Affinity purified
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Application
Western blot, Immunohistochemistry
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Application And Usages
Western blotting (0.5-4 µg/ml), immunocytochemistry (10-20µg/ml). However, the optimal conditions should be determined individually. The antibody detects primarily NFkB p65 in samples of human, mouse, and rat origins.
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Description
NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
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French translation
anticorps
