Annexin V-FITC

• Catalog number
  ANXVF-200T
• Price
  Please ask
• Size
  200 TEST

• Description
  Annexin V-FITC is a product that reacts with AnnexinV intracellular protein links, this reagent was FITC conjugated and reported for use in flow cytometric analysis to identify Apoptosis in targeted cells, this reagent interacting alone can't make difference between apoptic cells and necrotic, so we recomment the use of Annexin V Apoptosis Detection Kit (PI or 7AAD).
• Other apoptosis tools
• Other name
  Annexin A5
• Application
  Flow Cytometry Method
• Conjugate
  FITC
• Reactivity
  All mammalian
• Formulation
  Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
• Expiration
  After 1 year
• Storage
  2-8°C, store in dark, do not freeze
• Remark
  For Recherche Use Only.
• Additional description
  This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Immunostep has more cell death kits and antibodies.
• Properties
  This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Annexin  
• Gene symbol
  ANXA5
• Short name
  Annexin V-FITC
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  Annexin V-fluorecein
• Alternative technique
  fluorescine

Gene info

• Identity
  HGNC:543
• Gene
  ANXA5
• Long gene name
  annexin A5
• Synonyms gene
  • ENX2
  • ANX5
• Synonyms
  • CPB-I
  • PAP-I
  • VAC-alph
  • RPRGL3
• Synonyms name
  • annexin V
  • Calphobindin I
  • Placental anticoagulant protein I
  • Vascular anticoagulant-alpha
  • endonexin II
• GenBank acession
  • U05770
• Locus
  4q27
• Discovery year
  1989-05-31
• Entrez gene record
  308
• Pubmed identfication
  • 2960376
  • 14678296
• RefSeq identity
  • NM_001154
• Classification
  • Annexins
• VEGA ID
  OTTHUMG00000133034
• Locus Specific Databases
  • LRG_606

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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