PDGF-AB, human recombinant
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Catalog number
4485-10
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Price
Please ask
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Size
10 ug
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Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AB.
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Alternative_names
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AB, Beta platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1
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Description
A growth factor that regulates cell growth and division
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Purity
≥98%
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Biological activity
The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of >1 x 10⁶ units/mg.
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Results
< 1 ng/ml
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Molecular Weight
25.5 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Lyophilized with no additives
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Reconstitution Instructions
Reconstitute in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
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Background Information
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
PDGFA, QRICH1
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Short name
PDGF-AB, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
PDGF-Antibody, H. sapiens Rec.
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Alternative technique
rec
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Gene info
Gene info
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Identity
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Gene
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Long gene name
glutamine rich 1
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Synonyms
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Locus
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Discovery year
2005-12-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspase recruitment domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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