IgG1 Isotype Control Fc fusion protein FITC

• Catalog number
  35R-0007
• Price
  Please ask
• Size
  50 µg

• Category
  Biological Reagents
• Antibody Subtype
  Isotype Controls
• Area of research
  Immunology
• Raised in
  Rat
• Isotypes
  IgG1 kappa
• Concentration
  500 ug/ml
• Form Buffer
  Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
• Storage
  Store at 2-8 deg C. Do not freeze. Protect from light.
• Shipping conditions
  Blue Ice
• Tested for
  FC
• Assay Information
  control has been tested by immunofluorescent staining and flow cytometric analysis of mouse splenocyte suspensions and can be used at the same concentration as the experimental antibody
• URL
• More info
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
• Properties
  This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Anti-FITC Antibody
• Group
  isotypes

• Gene target
  IgG1   protein  
• Short name
  IgG1 Isotype Control Fc protein FITC
• Technique
  Control, Isotype, FITC, FC, controls, Fluorescein, Isotypes and controls
• Isotype
  IgG1
• Label
  FITC
• Alternative name
  Rat monoclonal IgG1 Isotype Control Fc fusion protein (FITC)
• Alternative technique
  control, controls, fluorescine
• Tissue
  control

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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