IgG1 Isotype Control Fc fusion protein biotin

  • Catalog number
    35R-0022
  • Price
    Please ask
  • Size
    50 µg
  • Category
    Biological Reagents
  • Antibody Subtype
    Isotype Controls
  • Area of research
    Immunology
  • Raised in
    Rat
  • Isotypes
    IgG1 kappa
  • Concentration
    500 ug/ml
  • Form Buffer
    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
  • Storage
    Store at 2-8 deg C. Do not freeze. Protect from light.
  • Shipping conditions
    Blue Ice
  • Tested for
    FC
  • Usage Recommendations
    FC: ≤ 0.5 ug/test
  • Assay Information
    A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
  • URL
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Biotinylated
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
  • Group
    isotypes
  • Gene target
    IgG1   protein  
  • Short name
    IgG1 Isotype Control Fc protein
  • Technique
    Control, Isotype, FC, controls, Isotypes and controls
  • Isotype
    IgG1
  • Label
    Biotin
  • Alternative name
    Rat monoclonal IgG1 Isotype Control Fc fusion protein (biotin)
  • Alternative technique
    control, controls
  • Tissue
    control
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