CUL2 Antibody Cullin-2

• Catalog number
  R30977
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  CUL2 Cullin-2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the CUL2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Cullin 2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q13617
• Purity
  Antigen affinity
• Description
  Cullin-2 is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, it was shown that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, Clifford et al.(1999) mapped the gene to 10p11.2-p11.1, a region reported to show loss of heterozygosity (LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. Pause et al.(1997) suggested that CUL2 is a candidate tumor suppressor gene, as has been proposed for CUL1 (603134). Lonergan et al.(1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL. CUL2 is one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere.
• Immunogen
  An amino acid sequence from the middle region of human Cullin-2 (HECQQRMVADHLQFLHA) was used as the immunogen for this CUL2 antibody.
• Storage
  After reconstitution, the CUL2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CUL2   Cullin-2  
• Gene symbol
  CUL2
• Short name
  Anti-CUL2 Cullin-2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to CUL2 Cullin-2
• Alternative technique
  antibodies
• Alternative to gene target
  cullin 2, CUL2 and IDBG-69264 and ENSG00000108094 and 8453, protein complex binding, nuclei, Cul2 and IDBG-127755 and ENSMUSG00000024231 and 71745, CUL2 and IDBG-637219 and ENSBTAG00000007133 and 535219

Gene info

• Identity
  HGNC:2552
• Gene
  CUL2
• Long gene name
  cullin 2
• GenBank acession
  • U83410
• Locus
  10p11.21
• Discovery year
  1998-10-29
• Entrez gene record
  8453
• Pubmed identfication
  • 8681378
• RefSeq identity
  • NM_003591
• Classification
  • Cullins
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000017950

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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