BDNF, human recombinant

  • Catalog number
    4004-250
  • Price
    Please ask
  • Size
    250 ug
  • Synonyms
    Brain Derived Neurotrophic Factor
  • Alternative_names
    Brain-derived neurotrophic factor, Short name=BDNF, Abrineurin
  • Description
    A potent neurotrophic factor supporting the growth and survival of nerve/glial cells
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥98%
  • Assay
    SDS-PAGE
  • Purity
    ≥98%
  • Biological activity
    The ED₅₀ determined by its ability to stimulate chick E8 neurite outgrowth was found to be 0.1-1.0 ng/ml.
  • Results
    0.1-1.0 ng/ml.
  • Molecular Weight
    27.0 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized protein
  • Physical form description
    Sterile filtered and lyophilized with no additives
  • Reconstitution Instructions
    Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
  • Background Information
    Human Brain-Derived Neurotrophic Factor (BDGF) is a potent neurotrophic factor that supports the growth and survivability of nerve and glial cells. The active form of BDNF is a 27.0 kDa dimer, formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    BDNF  
  • Gene symbol
    BDNF, BDNF-AS
  • Short name
    BDNF, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    brain-derived neurotrophic factor, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    brain-derived neurotrophic factor, ANON2 and BULN2, BDNF and IDBG-36817 and ENSG00000176697 and 627, growth factor activity, Extracellular, Bdnf and IDBG-195506 and ENSMUSG00000048482 and 12064, BDNF and IDBG-636102 and ENSBTAG00000008134 and 617701
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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