BDNF, human recombinant
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Catalog number4004-250
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PricePlease ask
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Size250 ug
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SynonymsBrain Derived Neurotrophic Factor
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Alternative_namesBrain-derived neurotrophic factor, Short name=BDNF, Abrineurin
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DescriptionA potent neurotrophic factor supporting the growth and survival of nerve/glial cells
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityThe ED₅₀ determined by its ability to stimulate chick E8 neurite outgrowth was found to be 0.1-1.0 ng/ml.
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Results0.1-1.0 ng/ml.
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Molecular Weight27.0 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionSterile filtered and lyophilized with no additives
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Reconstitution InstructionsReconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
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Background InformationHuman Brain-Derived Neurotrophic Factor (BDGF) is a potent neurotrophic factor that supports the growth and survivability of nerve and glial cells. The active form of BDNF is a 27.0 kDa dimer, formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolBDNF, BDNF-AS
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Short nameBDNF, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namebrain-derived neurotrophic factor, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetbrain-derived neurotrophic factor, ANON2 and BULN2, BDNF and IDBG-36817 and ENSG00000176697 and 627, growth factor activity, Extracellular, Bdnf and IDBG-195506 and ENSMUSG00000048482 and 12064, BDNF and IDBG-636102 and ENSBTAG00000008134 and 617701
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Gene info
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Identity
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Gene
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Long gene namebrain derived neurotrophic factor
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-01-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neurotrophins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameBDNF antisense RNA
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Synonyms gene
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Synonyms gene name
- BDNF opposite strand (non-protein coding)
- BDNF antisense RNA (non-protein coding)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-02-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data