PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit

• Catalog number
  K673-100
• Price
  Please ask
• Size
  100 assays

• Description
  Only Kit on the market to measure DHAP
• Summary
  • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Application- Measurement of Dhydroxyacetone Phosphate in various tissues/cells • Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis • Mechanistic study of human TPI deficiency
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Applications
  • Measurement of Dhydroxyacetone Phosphate in various tissues/cells • Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis • Mechanistic study of human TPI deficiency
• Sample Type
  Animal tissues: e.g., liver, muscle etc. • Cell culture: adherent or suspension cells • Biological fluids: serum, plasma etc.
• Features Benefits
  • Simple, Sensitive & Rapid protocol • Highly-Sensitive: Detects as low as 0.5 µM DHAP
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes. Biovision’s PicoProbeTM Dihydroxyacetone Phosphate Assay kit is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, TPI converts DHAP to GAP that undergoes a series of reaction and reduces PicoProbe™ to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of Dihydroxyacetone Phosphate. This simple, sensitive, and easy to use assay kit can detect Dihydroxyacetone Phosphate as low as 0.5 µM in a variety of samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Conjugate
  Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit

• Gene target
  Dihydroxyacetone   Phosphate   DHAP   Fluorometric   Kit  
• Short name
  ™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  PicoProbe™ Dihydroxyacetone Phosphate (DHAP) fluorescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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