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Clone
4G11-C7
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Immunogen
Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1
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Antibody s full description
Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa Antibody, Clone: 4G11-C7: RPE
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
DNA Mtase Antibody, DNMT Antibody, MCMT Antibody, DNA methyltransferase 1 Antibody, AIM Antibody, CXXC9 Antibody, DNMT Antibody, DNA (cytosine-5)-methyltransferase 1 Antibody, CXXC-type zinc finger protein 9 Antibody, DNA methyltransferase HsaI Antibody
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Raised in
Mouse
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Antibody s target
DNMT1
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Primary research fields
Cell Signaling, Post-translational Modifications, Organelle Markers
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Brandname
None
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Antibodies applications
WB, IHC, IP, ChIP
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Antibody s reactivity
Human, Mouse, Fish, Zebrafish (Danio rerio)
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Antibody s dilutions
WB (1:500), IHC (1:500); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~180kDa. It will cross-react with mouse DNMT1.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
2 µg/ml of SMC-201 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
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Bibliography
1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.
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Released date
1-Aug-2011
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_001370
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Gene number
1786
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Protein number
P26358
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Antibody s datasheet
Contact our support service
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Representative figure legend
Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 4G11-C7 (SMC-201). Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (SMC-201) at 1:1000. Mouse Anti-DNMT1 Antibody [4G11-C7] used in Western Blot (WB) on Human H1299 cell lysate (SMC-201)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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Gene target
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Gene symbol
C7, DNMT1, CXCL10, ERVK-6
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Short name
Mouse Anti- DNMT1 Monoclonal IgG1 Kappa, Clone 4G11-C7
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Technique
anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG1 Kappa, IgG1
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Label
RPE
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Species
Human, Humans, Mouses
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Alternative name
Mouse Antibody toHuman DNMT1 monoclonal IgG1 Kappa, clonality 4G11-complement component 7
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Alternative technique
murine, antibodies
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Alternative to gene target
complement component 7, C7 and IDBG-18519 and ENSG00000112936 and 730, protein binding, Extracellular, C7 and IDBG-259737 and ENSMUSG00000079105 and 109828, BT.69954 and IDBG-629869 and ENSBTAG00000011766 and 507339
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Clone name
4G11-C7
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