Mycobacterium avium F420-0:gamma-glutamyl ligase (cofE) -E. coli

Long name

Recombinant Mycobacterium avium F420-0:gamma-glutamyl ligase (cofE)
Alternative names

F420-0--gamma-glutamyl ligase; Coenzyme F420:L-glutamate ligase; F420-0--gamma-glutamyl ligase; Coenzyme F420-0:L-glutamate ligase; Coenzyme F420-1:gamma-L-glutamate ligase
Gene name

cofE
Other gene names

MAV_4225; fbiB;
General description

F420-0:gamma-glutamyl ligase(cofE) is a recombinant protein expressed in E. coli. The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.
Product category

Recombinant Proteins
Expression system

E. coli
Available also expressed in

E Coli ; Yeast ; Baculovirus ; Mammalian Cell
Purity

Greater than 90% (determined by SDS-PAGE)
Form

Lyophilized protein
Storage

This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.
Applications

This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.

Gene target

Mycobacterium avium F420-0 gamma-glutamyl ligase cofE
Gene symbol

IGKV1OR2-0, SNORD112, H1-0
Short name

F420-0:gamma-glutamyl ligase(cofE)
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. MBS Recombinant supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Species

E. coli, E. coli
Alternative name

Mycobacterium avium F420-0:g-glutamyl ligase (cofE) -E. coli
Alternative technique

escherichia
Disease

mycobacterium

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data