Rabbit Anti-Human Collagen III Polyclonal: Streptavidin

• Catalog number
  SPC-1275D-STR
• Price
  Please ask
• Size
  100 µl

• Immunogen
  Synthesized peptide derived from internal of human collagen III.
• Antibody s target
  Human Collagen III
• Antibody s full description
  Rabbit Anti-Human Collagen III Polyclonal
• Primary research fields
  Cell Signaling, Extracellular Matrix (ECM), Collagen, Stem Cells, Mesenchymal Stem Cells, Osteogenesis, Stem Cells, Mesenchymal Stem Cells, Chondrogenesis
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  CO3A1_HUMAN Antibody, Collagen alpha 1(III) chain Antibody, Collagen III alpha 1 chain precursor Antibody, Collagen type III alpha 1 Antibody, EDS4A Antibody, Type III collagen Antibody
• Verified applications
  IHC, ICC/IF, ELISA
• Raised in
  Rabbit
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s recommended dilutions for use
  IHC (1:100), ICC/IF (1:500), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Peptide Affinity Purified
• Recommended buffer for storage
  PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
• Antibody s concentration
  See included datasheet or contact our support service
• Antibody s specificity
  Detects ~138kDa, endogenous levels of total collagen III protein.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  A 1:100 dilution of SPC-1275 was sufficient for detection of Collagen III in human pancreas tissue using Goat Anti-Rabbit IgG as the secondary antibody.
• Antibody in cell
  Extracellular matrix
• Tissue specificity
  Soft connective tissues
• Scientific context
  Type-III collagen is the second most abundant form of collagen in the human body and can be found in intestinal walls, muscles, blood vessels and with collage type-I. Collagen Type-I and Type-III can be mixed together to create supplements for the skin, bone and muscles.
• Bibliography
  1. Janeczko R.A., Ramirez F. (1989). Nucleic Acids Res. 17(16): 6742. 2. Superti-Furga A., Gugler E., Gitzelmann R., Steinmann B. (1988). J Biol Chem. 263(13): 6226–32. 3. "Entrez Gene: COL3A1 collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant)".
• Released date
  6/Sep/2016
• NCBI number
  NP_000081.1
• Gene number
  1281
• Protein number
  P02461
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
  more | details
• Representative figure legend
  Immunohistochemistry analysis using Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275). Tissue: Pancreas Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275). Tissue: HeLa. Species: Human. Primary Antibody: Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-Collagen III Antibody used in Immunohistochemistry (IHC) on Pancreas Tissue (SPC-1275) | Rabbit Anti-Collagen III Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HeLa (SPC-1275)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Gene
  Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  Collagen   III   Streptavidin  
• Gene symbol
  CPLX3, MGAT3, MGST3, P4HA3, CASP5, THAP4, IGHVIII-82, MT-CSB3, IGHVIII-67-4, IGHVIII-76-1
• Short name
  Rabbit Anti- Collagen III Polyclonal: Streptavidin
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG
• Label
  Streptavidin
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman Collagen III polyclonal: biotinilated-binding streptavidin
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:27652
• Gene
  CPLX3
• Long gene name
  complexin 3
• Synonyms
  • CPX-III
• GenBank acession
  • BC018026
• Locus
  15q24.1
• Discovery year
  2005-08-02
• Entrez gene record
  594855
• Pubmed identfication
  • 15911881
• RefSeq identity
  • NM_001030005
• VEGA ID
  OTTHUMG00000142816

Gene info

• Identity
  HGNC:7046
• Gene
  MGAT3
• Long gene name
  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• Synonyms gene name
  • mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• Synonyms
  • GNT-III
• GenBank acession
  • D13789
• Locus
  22q13.1
• Discovery year
  1993-08-27
• Entrez gene record
  4248
• Pubmed identfication
  • 8370666
• RefSeq identity
  • NM_002409
• Classification
  • Mannosyl-glycoprotein N-acetylglucosaminyltransferases
• VEGA ID
  OTTHUMG00000030185

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:30135
• Gene
  P4HA3
• Long gene name
  prolyl 4-hydroxylase subunit alpha 3
• Synonyms gene name
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
  • prolyl 4-hydroxylase, alpha polypeptide III
• Synonyms
  • C-P4Halpha(III)
• Synonyms name
  • collagen prolyl 4-hydroxylase alpha(III)
• GenBank acession
  • AY327887
• Locus
  11q13.4
• Discovery year
  2004-05-12
• Entrez gene record
  283208
• Pubmed identfication
  • 14500733
• RefSeq identity
  • NM_182904
• VEGA ID
  OTTHUMG00000165254

Gene info

• Identity
  HGNC:1506
• Gene
  CASP5
• Long gene name
  caspase 5
• Synonyms gene name
  • caspase 5, apoptosis-related cysteine protease
  • caspase 5, apoptosis-related cysteine peptidase
• Synonyms
  • ICE(rel)III
• Locus
  11q22.3
• Discovery year
  1996-09-13
• Entrez gene record
  838
• Pubmed identfication
  • 7797592
  • 9250871
• RefSeq identity
  • NM_004347
• Classification
  • Caspases
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000048073

Gene info

• Identity
  HGNC:23187
• Gene
  THAP4
• Long gene name
  THAP domain containing 4
• Synonyms
  • CGI-36
  • Nb(III)
• Synonyms name
  • nitrobindin
• GenBank acession
  • AF258556
• Locus
  2q37.3
• Discovery year
  2003-10-08
• Entrez gene record
  51078
• Pubmed identfication
  • 12575992
  • 10810093
  • 30524950
• RefSeq identity
  • NM_015963
• Classification
  • THAP domain containing
• VEGA ID
  OTTHUMG00000133410

Gene info

• Identity
  HGNC:5709
• Gene
  IGHVIII-82
• Long gene name
  immunoglobulin heavy variable (III)-82 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-82
  • immunoglobulin heavy variable (III)-82 pseudogene
• Synonyms
  • IGHV(III)-82
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28338
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151732

Gene info

• Identity
  HGNC:7426
• Gene
  MT-CSB3
• Long gene name
  mitochondrially encoded conserved sequence block III
• Synonyms gene
  • MTCSB3
• Synonyms gene name
  • conserved sequence block III
• Synonyms
  • CSB-III
• Locus
  mitochondria
• Discovery year
  1989-10-11
• Pubmed identfication
  • 2982153
• Classification
  • Mitochondrially encoded regions

Gene info

• Identity
  HGNC:5707
• Gene
  IGHVIII-67-4
• Long gene name
  immunoglobulin heavy variable (III)-67-4 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-67-4
  • immunoglobulin heavy variable (III)-67-4 pseudogene
• Synonyms
  • IGHV(III)-67-4
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28340
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151874

Gene info

• Identity
  HGNC:5708
• Gene
  IGHVIII-76-1
• Long gene name
  immunoglobulin heavy variable (III)-76-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-76-1
  • immunoglobulin heavy variable (III)-76-1 pseudogene
• Synonyms
  • IGHV(III)-76-1
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28339
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151872

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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