Rabbit Anti-Human Collagen III Polyclonal: FITC
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Catalog numberSPC-1275D-FITC
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PricePlease ask
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Size100 µl
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ImmunogenSynthesized peptide derived from internal of human collagen III.
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Antibody s targetHuman Collagen III
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Antibody s full descriptionRabbit Anti-Human Collagen III Polyclonal
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Primary research fieldsCell Signaling, Extracellular Matrix (ECM), Collagen, Stem Cells, Mesenchymal Stem Cells, Osteogenesis, Stem Cells, Mesenchymal Stem Cells, Chondrogenesis
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameCO3A1_HUMAN Antibody, Collagen alpha 1(III) chain Antibody, Collagen III alpha 1 chain precursor Antibody, Collagen type III alpha 1 Antibody, EDS4A Antibody, Type III collagen Antibody
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Verified applicationsIHC, ICC/IF, ELISA
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useIHC (1:100), ICC/IF (1:500), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects ~138kDa, endogenous levels of total collagen III protein.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:100 dilution of SPC-1275 was sufficient for detection of Collagen III in human pancreas tissue using Goat Anti-Rabbit IgG as the secondary antibody.
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Antibody in cellExtracellular matrix
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Tissue specificitySoft connective tissues
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Scientific contextType-III collagen is the second most abundant form of collagen in the human body and can be found in intestinal walls, muscles, blood vessels and with collage type-I. Collagen Type-I and Type-III can be mixed together to create supplements for the skin, bone and muscles.
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Bibliography1. Janeczko R.A., Ramirez F. (1989). Nucleic Acids Res. 17(16): 6742. 2. Superti-Furga A., Gugler E., Gitzelmann R., Steinmann B. (1988). J Biol Chem. 263(13): 6226–32. 3. "Entrez Gene: COL3A1 collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant)".
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Released date6/Sep/2016
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NCBI numberNP_000081.1
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Gene number1281
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Protein numberP02461
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275). Tissue: Pancreas Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275). Tissue: HeLa. Species: Human. Primary Antibody: Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-Collagen III Antibody used in Immunohistochemistry (IHC) on Pancreas Tissue (SPC-1275) | Rabbit Anti-Collagen III Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HeLa (SPC-1275)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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GeneCollagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
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PropertiesThis StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolCPLX3, MGAT3, MGST3, P4HA3, CASP5, THAP4, IGHVIII-82, MT-CSB3, IGHVIII-67-4, IGHVIII-76-1
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Short nameRabbit Anti- Collagen III Polyclonal: FITC
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG
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LabelFITC
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman Collagen III polyclonal: fluorecein
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Alternative techniquepolyclonals, rabbit-anti, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene namecomplexin 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
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Synonyms gene name
- mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprolyl 4-hydroxylase subunit alpha 3
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Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
- prolyl 4-hydroxylase, alpha polypeptide III
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-05-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 5
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Synonyms gene name
- caspase 5, apoptosis-related cysteine protease
- caspase 5, apoptosis-related cysteine peptidase
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Synonyms
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Locus
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Discovery year1996-09-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Caspase recruitment domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTHAP domain containing 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- THAP domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-82 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-82
- immunoglobulin heavy variable (III)-82 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrially encoded conserved sequence block III
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Synonyms gene
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Synonyms gene name
- conserved sequence block III
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Synonyms
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Locus
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Discovery year1989-10-11
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Pubmed identfication
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Classification
- Mitochondrially encoded regions
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-67-4 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-67-4
- immunoglobulin heavy variable (III)-67-4 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-76-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-76-1
- immunoglobulin heavy variable (III)-76-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
