Conjugated Primary Antibodies
Rabbit (Oryctolagus cuniculus)
This is a highly specific antibody against Bub1.
1ug per 1ul
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Bub1
Gene ID Number
Human, Mouse, Rat
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
Purified by Protein A.
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
BUB1A; BUB1L; hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; BUB1
Immunoglobulin G (IgG)
Alternative to gene target
budding uninhibited by benzimidazoles 1 homolog (yeast), BUB1A and BUB1L and hBUB1, BUB1 and IDBG-65670 and ENSG00000169679 and 699, transferase activity, nuclei, Bub1 and IDBG-205152 and ENSMUSG00000027379 and 12235, BUB1 and IDBG-628990 and ENSBTAG00000021181 and 514777