CD45RA FITC Monoclonal Antibody (Clone HI100)

• Catalog number
  6959-100
• Price
  Please ask
• Size
  100 tests

• Antibody Target
  CD45RA
• Target Alternative Name
  CD45RA
• Antibody Type
  Monoclonal
• Clone
  HI100
• Species Reactivities
  Human
• Immunogen Sequence
  Human CD45RA
• Accession
  P08575
• Gene Id
  5788
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  Phosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.
• Purification
  Affinity chromatography.
• Application
  Flow cytometry
• Positive Control
  FACS: Human peripheral blood lymphocytes
• Application And Usages
  Flow (Cell Surface): 5 µl/1x10^6 cells, Volume per test: 5 µl (1 µg).
• Description
  CD45, a transmembrane multifunctional glycoprotein, is a member of Type I receptor-linked PTPase family and is expressed as multiple isoforms due to alternative splicing. Expression of these isoforms is highly regulated and shift in this expression determines T-cell activation. CD45RB consists of exon B and is predominantly expressed in naïve T-cells secreting IL-2. Its expression is low in primed/memory T cells, cells that express Th2 cytokines such as IL-4 and IL-10 and population of T-cells with regulatory function. Immunotherapy with CD45RB antibody is being widely studied in transplantation and vaccination. CD45 antibodies are commonly used to identify tumors of lymphoid origin. The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes. The CD45RA antigen is expressed by naïve and activated T lymphocytes. The HI100 monoclonal antibody is used as a phenotypic marker to discriminate T lymphocytes subsets.
• Storage Condition
  4°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD45RA   Monoclonal   HI100  
• Short name
  CD45RA FITC Monoclonal Antibody (Clone HI100)
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
• Isotype
  Mouse IgG2a, Kappa
• Label
  FITC
• Alternative name
  CD45RA fluorecein monoclonal (antibody to-) (clonality HI100)
• Alternative technique
  antibodies, fluorescine
• Clone name
  HI100

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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