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Immunogen
Synthesized peptide derived from internal of human PINX1.
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Antibody s target
Human PINX1
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Antibody s full description
Rabbit Anti-Human PINX1 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
67-11-3 protein antibody, FLJ20565 antibody, Hepatocellular carcinoma-related putative tumor suppressor antibody, Liver-related putative tumor suppressor antibody, LPTL antibody, LPTS antibody, MGC8850 antibody, OTTHUMP00000224984 antibody, OTTHUMP00000224985 antibody, OTTHUMP00000224986 antibody, OTTHUMP00000225053 antibody, OTTHUMP00000225054 antibody, PIN2 interacting protein 1 antibody, Pin2-interacting protein X1 antibody, PIN2/TERF1-interacting telomerase inhibitor 1 antibody, PINX1 antibody, PINX1_HUMAN antibody, Protein 67-11-3 antibody, TRF1-interacting protein 1 antibody
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Verified applications
IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
IHC (1:100), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total PINX1 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:100 dilution of SPC-1330 was sufficient for detection of PINX1 in human heart tissue using Goat Anti-Rabbit IgG as the secondary antibody.
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Antibody in cell
Nucleus, Chromosome
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
PIN2/TERF1-interacting telomerase inhibitor 1, also known as PINX1, is a human gene. It mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres, inhibits telomerase activity. it may also inhibit cell proliferation and act as tumor suppressor. Over-expression of PINX1 results in decreased telomerase activity, telomere shortening, and induction of crisis. Reduction of PINX1 leads to an increase in telomerase activity and elongation of telomeres. PINX1 differs from other proteins that regulate telomere length in that it acts on telomerase while other proteins adjust telomere length without affecting telomerase activity.
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Bibliography
1. Song H. et al. (2004) Biochem. Biophys. Res. Commun. (United States) 316 (4): 1116–23. 2. Soohoo, C.Y. (2010) Journal of Biological Chemistry. 3. Yoo, J.E. (2009) Journal of Molecular Biology. 4. Zhou, X.Z. (2011) Journal of Clinical Investigation.
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Released date
6/Sep/2016
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NCBI number
NP_060354.4
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Gene number
54984
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Protein number
Q96BK5
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-PINX1 Polyclonal Antibody (SPC-1330). Tissue: Heart Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-PINX1 Polyclonal Antibody (SPC-1330) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-PINX1 Antibody used in Immunohistochemistry (IHC) on Heart Tissue (SPC-1330)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
