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Gene info
DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.
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Gene names
PRKDC, DNA-PK, DNAPK, DNPK1, HYRC, HYRC1, p350, Scid, XRCC7, DNA-PK catalytic subunit, DNA-PKcs, p460
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Antibodie s label
unlabelled serum
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Purity
Immunoaffinity Chromatography
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Ab raised in
Rabbit
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Type
Polyclonal
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Spicific to
pThr2609
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Antigen
PRKDC antibody was made against aa 2604 - 2614 of PRKDC (Human).
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Immuno application
ELISA, Western Blot, immuno-histo-chemistry
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Antibody advice
PRKDC antibody can be used in ELISA starting at 1:4000 - 1:16000, Western Blot starting at 1:500 - 1:3000, and immunohistochemistry starting at 10 - 20 ug/mL.
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Suspention pH azide
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
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Keep at
Store PRKDC antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
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Works with
Chimpanzee, Human
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Omim nr
38258929
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Ncbi nr
P78527
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Vial with antibody
in solution
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NCBI GENE nr
5591
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GENE symbol
PRKDC
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Antigene name
protein kinase, DNA-activated, catalytic polypeptide
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Source
Homo sapiens
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Protein nr
P78527
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
PRKDC
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Short name
anti-PRKDC Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toPRKDC (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
protein kinase, DNA-activated, catalytic polypeptide, DNA-PKcs and DNAPK and DNPK1 and HYRC and HYRC1 and IMD26 and p350 and XRCC7, PRKDC and IDBG-408612 and ENSG00000253729 and 5591, transferase activity, nuclei, Prkdc and IDBG-135771 and ENSMUSG00000022672 and 19090, PRKDC and IDBG-644289 and ENSBTAG00000017019 and 512740
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