Protein A (Lyophilized)

  • Catalog number
    6500B-100
  • Price
    Please ask
  • Size
    100 mg
  • Synonyms
    Protein A
  • Alternative_names
    Protein A
  • Description
    Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥97%
  • Assay
    SDS-PAGE
  • Purity
    ≥97%
  • Binding Capacity
    hIgG BINDING: >95%
  • Molecular Weight
    39.21 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized powder
  • Reconstitution Instructions
    Reconstitute in H₂O
  • Background Information
    The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • About
    The term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen.
  • Gene target
    Protein  
  • Gene symbol
    CENPA, VAPA, AKAP10
  • Short name
    Protein A ( )
  • Alternative name
    Protein A (Lyophilized)
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    VAMP associated protein A
  • Synonyms gene name
    • VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
    • VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
  • Synonyms
  • Locus
  • Discovery year
    1999-03-19
  • Entrez gene record
  • Pubmed identfication
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.
  • Tree numbers
    • E01.370.225.500.620.760.160.260
    • E01.370.225.750.600.760.160.260
    • E02.792.156.260
    • E05.200.500.620.760.160.260
    • E05.200.750.600.760.160.260
    • E05.760.156.260
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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