Protein A (Lyophilized)
-
Catalog number6500B-100
-
PricePlease ask
-
Size100 mg
-
-
SynonymsProtein A
-
Alternative_namesProtein A
-
DescriptionGenetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
-
RecombinantYes
-
SourceE. coli
-
Purity by SDS PAGE≥97%
-
AssaySDS-PAGE
-
Purity≥97%
-
Binding CapacityhIgG BINDING: >95%
-
Molecular Weight39.21 kDa
-
Storage Temp-20°C
-
ShippingGel Pack
-
Shelf Life12 months
-
AppearanceLyophilized
-
Physical form descriptionLyophilized powder
-
Reconstitution InstructionsReconstitute in H₂O
-
Background InformationThe recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
AboutThe term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen.
-
Gene target
-
Gene symbolCENPA, VAPA, AKAP10
-
Short nameProtein A ( )
-
Alternative nameProtein A (Lyophilized)
-
Gene info
-
Identity
-
Gene
-
Long gene namecentromere protein A
-
Synonyms gene name
- centromere protein A (17kD)
- centromere protein A, 17kDa
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1988-05-31
-
Entrez gene record
-
RefSeq identity
-
Classification
- Constitutive centromere associated network
- H3 histones
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameVAMP associated protein A
-
Synonyms gene name
- VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
- VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
-
Synonyms
-
Locus
-
Discovery year1999-03-19
-
Entrez gene record
-
Pubmed identfication
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameA-kinase anchoring protein 10
-
Synonyms gene name
- A kinase (PRKA) anchor protein 10
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1999-09-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- A-kinase anchoring proteins
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.
-
Tree numbers
- E01.370.225.500.620.760.160.260
- E01.370.225.750.600.760.160.260
- E02.792.156.260
- E05.200.500.620.760.160.260
- E05.200.750.600.760.160.260
- E05.760.156.260
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data