Goat, F(ab)'2 fragment, ALP conjugated min. cross-reactivity to bovine, horse, human, pig or rabbit serum protein
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Catalog numberAS16 3440
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PricePlease ask
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Size0.5 mg
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Available ordering formatLiquid
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ImmunogenPurified Mouse IgG, whole molecule
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Raised inGoat
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionStore at 2-8 °C.
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Verified applicationsFlow cytometry (FACS), Immunomicroscopy (IM)
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Connected productsother anti-mouse secondary antibodies
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Recommended dilutions for use1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected Ğ°pparentsee datasheet
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Verified reactivityMouse IgG (H&L), F(ab)'2 Fragment
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Possible reactivityto be determined
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No reactivityto be determined
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Supplementary informationContact Gentaur support center at [email protected]
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ReferencesTo be added when available, antibody released in April 2016.
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Scientific contextGoat anti-Mouse IgG (H&L), F(ab)'2 fragment, ALP conjugated min. cross-reactivity to bovine, horse, human, pig or rabbit serum protein is a secondary antibody conjugated to ALP, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
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NotesAntibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free.Based on IEP, this antibody reacts with: - heavy (gamma) chains on mouse IgG - light chains on all mouse immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin mouse serum immunoglobulins - serum proteins from bovine, horse, human rabbit or swine .
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Protein numberRefer to NCBI
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TAIR numberRefer to NCBI
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Latin nameCapra aegagrus hircus, Oryctolagus cuniculus
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PropertiesHorse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutPigs and the smaller guinea pigs are frequent used as models for humans. Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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DescriptionAzide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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GroupSera
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Gene target
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Gene symbolPDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
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Short nameGoat, F(ab)'2 , ALP conjugated min. cross-reactivity , , , rabbit serum protein
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TechniqueRabbit, serum, Goat, antibody Conjugates, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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IsotypeAntibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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SpeciesPig, Bos Taurus genes are available form the Bovine Genome database. Goats, Horses, Humans, Pigs and Guinea pigs
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Alternative namecaprine, F(Antibody)'2 fragment, ALP coupled min. cross-This (antibody to-) recognizes to bovine, horse, H. sapiens, swine and production species: rabbit blood serum protein
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Alternative techniquerabbit-anti, sera, caprine
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Tissueserum
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Gene info
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Identity
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Gene
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Long gene namePDZ and LIM domain 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
- LIM domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameasparaginase and isoaspartyl peptidase 1
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Synonyms gene name
- asparaginase like 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameattractin like 1
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Synonyms gene name
- attractin-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesecretory leukocyte peptidase inhibitor
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Synonyms gene name
- secretory leukocyte protease inhibitor (antileukoproteinase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-24
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Entrez gene record
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Pubmed identfication
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Classification
- WAP four-disulfide core domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 27
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 27
- chemokine (C-C motif) ligand 27
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-07-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data