Rabbit Luciferase antibody (FITC)
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Catalog number
60C-CR2029RAF
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Price
Please ask
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Size
500 ul
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Applications
ELISA, IHC
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Product Type
Primary Antibodies
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Product Subtype
Conjugated Polyclonal Antibodies
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Research Area
Proteases, Inhibitors, & Enzymes
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Immunogen
Luciferase antibody (FITC) was raised in rabbit using highly purified firefly luciferase as the immunogen.
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Specificity
Firefly
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Cross Reactivity
Specific for Firefly Luciferase (Photinus pyralis)
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Clone
NA
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Concentration
50 ug/ml
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Form Buffer
Affinity purified and supplied in PBS, pH 7.4, with 0.2% BSA and 0.05% NaN3.
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Storage
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping Info
Blue Ice
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Rabbit Luciferase antibody (FITC)
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Technique
Antibody, Rabbit, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Host
Rabbit, Rabbits
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Isotype
NA
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Label
luciferase
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Alternative name
Affinity purified Rabbit polyclonal Luciferase antibody (FITC)
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Alternative technique
antibodies, rabbit-anti, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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